B cells develop in the zebrafish pancreas

B cells develop in the zebrafish pancreas.

The zebrafish, with its transparent free-living embryo, is a useful organism for investigating early stages in lymphopoiesis. Previously, we showed that T cells differentiate in the thymus by day 4, but no sites for B cell differentiation were seen until 3 weeks. We report here that on day 4, we detect rearrangements of genes encoding B cell receptors in DNA extracted from whole fish. Also by d...

Regeneration of the Pancreas in Adult Zebrafish

OBJECTIVE Regenerating organs in diverse biological systems have provided clues to processes that can be harnessed to repair damaged tissue. Adult mammalian beta-cells have a limited capacity to regenerate, resulting in diabetes and lifelong reliance on insulin. Zebrafish have been used as a model for the regeneration of many organs. We demonstrate the regeneration of adult zebrafish pancreatic...

osteoclast-like cells in early zebrafish embryos

objective: genes involved in bone and tissue remodelling in the vertebrates include matrix metalloproteinase-9 (mmp-9), receptor activator of necrosis factor κ-β (rank), cathepsin-k (ctsk) and tartrate-resistant acid phosphatase (tracp). we examine whether these markers are expressed in cells of zebrafish embryos of 1-5 days post fertilization. we also examine adult scales, which are known to c...

Autoreactive T Cells B 1 Cells Promote Pancreas

Targeted ablation of beta cells in the embryonic zebrafish pancreas using E. coli nitroreductase

In order to generate a zebrafish model of beta cell regeneration, we have expressed an Escherichia coli gene called nfsB in the beta cells of embryonic zebrafish. This bacterial gene encodes a nitroreductase (NTR) enzyme, which can convert prodrugs such as metronidazole (Met) to cytotoxins. By fusing nfsB to mCherry, we can simultaneously render beta cells susceptible to prodrug and visualize M...